S. pneumo niae has approximately 10-16 identified CBPs (17,(148)(149)(150) including pneumococcal surface protein A (PspA), pneumococcal surface protein C (PspC), and LytA which are discussed
To address this question, we used pneumococcal genomic data to identify variants of 21 pneumococcal protein antigens present in S. pneumoniae carriage isolates. We then assessed the association between antibody titers and subsequent colonization with S. pneumoniae strains eing an antigen recognized by preexisting antibodies. Characterization of pneumococcal Ser/Thr protein To identify these StkP substrates we extracted proteins from the membrane fraction using trifluorethanol (TFE) and separated them using two-dimensional (2D) SDS-PAGE as described in the Methods. The protein spot corresponding to P40 was successfully resolved, and its phosphorylation was confirmed by immunoblotting (Fig. 6a).
1. J Immunol Methods. 2018 Sep;460:72-78. doi:10.1016/j.jim.2018.06.011. Epub 2018 Jun 20. Comparison of serological assays using pneumococcal proteins or polysaccharides for detection of Streptococcus pneumoniae infection in children with community-acquired pneumonia. Creactive protein is essential for innate resistance to Introduction. The physiological functions of human Creactive protein (CRP), named for its interaction with pneumococcal somatic Cpolysaccharide, in which it specifically binds the phosphocholine residues, 1 are not known because no deficiency or even structural variants of the protein have been described. There are some extremely rare coding polymorphisms of the CRP gene but the variant
Introduction. The physiological functions of human Creactive protein (CRP), named for its interaction with pneumococcal somatic Cpolysaccharide, in which it specifically binds the phosphocholine residues, 1 are not known because no deficiency or even structural variants of the protein have been described. There are some extremely rare coding polymorphisms of the CRP gene but the variant Detection of 23 Immunogenic Pneumococcal Proteins Identification of known pneumococcal proteins. In total, 20 clones contained pneumococcal DNA of previously identified genes. They contained eight different DNA inserts from six genes (Fig.1).Five of the deduced proteins were surface located and one (DnaK) was presumably of cytoplasmic origin.
Pneumococcal surface protein A (PspA) and pneumococcal surface adhesin A (PsaA) are virulence proteins showing good immunogenicity and protective effects against S. pneumoniae strains in mice. In this study, we eed the fusion protein PsaA-PspA, which consists of PsaA and the N-terminal region of PspA family 1 and 2, in Escherichia coli . Identication of Pneumococcal Surface Protein A as a Identication of Pneumococcal Surface Protein A as a Lactoferrin-Binding Protein of Streptococcus pneumoniae SVEN HAMMERSCHMIDT, GESINA BETHE, PETRA
Four pneumococcal genes ( phtA, phtB, phtD , and phtE ) encoding a novel family of homologous proteins (32 to 87% identity) were identified from the Streptococcus pneumoniae genomic sequence. These open reading frames were selected as potential vaccine candidates based upon their possession of hydrophobic leader sequences which presumably target these proteins to the bacterial cell surface. Identification and Characterization of a Novel Family of Four pneumococcal genes (phtA, phtB, phtD, and phtE) encoding a novel family of homologous proteins (32 to 87% identity) were identified from the Streptococcus pneumoniae genomic sequence.These open reading frames were selected as potential vaccine candidates based upon their possession of hydrophobic leader sequences which presumably target these proteins to the bacterial
Identification and characterization of a novel family of pneumococcal proteins that are protective against sepsis. Adamou JE(1), Heinrichs JH, Erwin AL, Walsh W, Gayle T, Dormitzer M, Dagan R, Brewah YA, Barren P, Lathigra R, Langermann S, Koenig S, Johnson S. Identification of Pneumococcal Surface Protein A as a In this study we demonstrated that 88% of the tested strains (mainly clinical isolates) bound hLf specifically. The characterization of the Lf-binding protein and its amino acid sequence analysis revealed that the pneumococcal surface protein A, PspA, is the binding component. Among the pneumococcal surface proteins, PspA has been studied in
searching for protein vaccine candidates [15,16]. In this study, we have screened a collection of pneumococcal clinical isolates from adults by defining its pan-surfome, (i.e. the whole set of eed surface proteins), in order to identify which proteins that have not been tested so far in animal models for protection against Identification of Potential New Protein Vaccine Candidates Jul 23, 2013 · In the search for pneumococcal surface proteins to be proposed as potential new vaccine candidates, we established a threshold for the presence of a protein in at least 50% of the analyzed clinical isolates; otherwise, a very stringent criterion would lead us to identify the already described protective antigens.
Mucosal or parenteral immunization with a killed unencapsulated pneumococcal whole cell antigen (WCA) with an adjuvant protects mice from colonization by a T H 17 CD4+ cell-mediated mechanism. Using preparative SDS gels, we separated the soluble proteins that compose the WCA in order to identify fractions that were immunogenic and protective. Identification of SP1683 as a pneumococcal protein that is 25 rows · Serotype-independent protein-based pneumococcal vaccines represent attractive alternatives to
Under conditions of phosphate excess, PhoR, Pst, and PhoU downregulate the PHO regulon. Screening of a library of pneumococcal mutants with defects in exported proteins revealed a putative two-component regulatory system, PnpR-PnpS, and a downstream ABC transporter, similar to the Pst system in E. coli including a gene encoding a PhoU protein. Identification of an atypical zinc metalloproteinase, ZmpC Closer examination of the primary structure revealed that the SP168 ZmpC lacks the canonical LPXTG motif - a signature typical of several surface proteins of gram-positive bacteria and of other pneumococcal zinc metalloproteinases.
Apr 17, 2018 · The majority of cellular proteins undergo rapid degradation and synthesis to minimize the toxic effect to cells and tissues and to guarantee normal cellular functions. It has been appreciated that proteins with longer half-lives exist in certain cells and tissues. Here we identify synaptic long-lived proteins by high-resolution mass spectrometry. Identification of pneumococcal proteins that are Four proteins, RodA, MreD, DivIVA and Spr0777, were identified by this approach. Together with PBP2b these proteins are essential for the normal function of the elongasome. Furthermore, our findings suggest that DivIVA, which was previously assigned as a divisomal protein, is required to correctly localize the elongasome at the negatively curved membrane region between the septal and lateral cell wall.
Identification of pneumococcal proteins that are functionally linked to penicillin-binding protein 2b (PBP2b). Straume D(1), Stamsås GA(1), Berg KH(1), Salehian Z(1), Håvarstein LS(1). Author information:(1)Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, NO-1432, Norway. Identification of pneumococcal proteins that are Sep 29, 2016 · Identification of pneumococcal proteins that are functionally linked to penicillinbinding protein 2b (PBP2b)
Identification of proteins in Streptococcus pneumoniae by reverse vaccinology and genetic diversity of these proteins in clinical isolates. Streptococcus pneumoniae is a major cause of morbidity and mortality worldwide. Virulence-associated proteins common and conserved among all capsular types now represent the best strategy to combat pneumococcal infections. Identification of the Major Protein Component of the During genetic transformation of Streptococcus pneumoniae , single strands from native donor DNA enter competent cells, where they associate with an unidentified protein with a molecular mass of 15 to 20 kDa to form the eclipse complex. Using Western blotting, we identify the principal protein cofractionating with donor DNA in this complex as SsbB.
In conclusion, we show here mechanisms of pneumococcal adaptation to a defined host compartment by a proteome-based approach. Further, this study provides the basis of a promising strategy for the identification of protein antigens critical for invasive disease caused by pneumococci and other meningeal pathogens. Interaction of pneumococcal histidine triad proteins with The pneumococcal histidine triad (Pht) proteins PhtA, PhtB, PhtD, and PhtE form a group of conserved pneumococcal surface proteins. Humans produce antibodies to Pht proteins upon exposure to pneumococcus, and immunization of mice has provided protective immunity against sepsis and pneumonia and reduced nasopharyngeal colonization.
Jan 01, 2015 · Introduction. Surface proteins of Streptococcus pneumoniae (the surfeome) play an important role in the interaction with the human host, as these are the primary points of contact with the host cells and the immune system. Many well-studied pneumococcal surface proteins will be discussed in other chapters. Here we will focus on a subset of surface proteins of pneumococcus, namely those Novel Protein-Based Pneumococcal Vaccines:Assessing the Non-serotype-specific protein-based pneumococcal vaccines have received extensive research focus due to the limitations of polysaccharide-based vaccines. Pneumococcal proteins (PnPs), universally eed among serotypes, may induce broader immune responses, stimulating humoral
Hypothesis:Two Streptococcus pneumoniae proteins, pneumococcal surface protein A (PspA) and pneumolysin (Ply), have functional and histopathologic effects on the inner ear. Background:Temporary or permanent sensorineural hearing loss is known to be a sequela of pneumococcal otitis media. Several pneumococcal proteins such as PspA and Ply have been shown to contribute to the Streptococcus pneumoniae:proteomics of surface proteins Two formulations of pneumococcal vaccines are currently available to prevent invasive disease in adults and children. However, these vaccines will not protect against the majority of Streptococcus pneumoniae serotypes. The use of highly conserved cell-wall-associated proteins in vaccines may circumv
Jan 01, 2015 · Ply was the first pneumococcal protein to be proposed as a vaccine antigen, as demonstrated by the finding that immunization of mice with highly purified toxin provided protection against intranasal challenge with virulent pneumococci . Ply antibody-mediated protection is presumed to be due largely to neutralization of free toxin. Identification of pneumococcal proteins that are Sep 29, 2016 · We succeeded in identifying four proteins that, when deleted or depleted, gave rise to CbpDhypersensitive strains, namely:RodA, MreD, DivIVA and Spr0777.